白色レグホーン雄鶏において100日令時に睾丸の急激な発達が起つた。鶏冠の発育は睾丸の発育とは軌を一にしなかつた。下垂体前葉の比体重値は100日令まで減少し,その後はほぼ一定値を示した。前葉のGTH力価(available hormone)は睾丸発育の急変期に著変が認められず,一方睾丸のGTHに対する感受性は同時期に高まつた。

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An experiment was carried out to induce the superovulation in mature rabbits (Japanese White Breed, more than 2 kg in body weight) by PMS and CG with progesterone pretreatment. The principal method is as follows; 1mg progesterone twice-daily for five days, subsequently 20 I.U. PMS twice-daily for five days followed by CG and mating. After two days' intermission, does were autopsied and the number of ovulation point, blood follicle, and follicle in the ovary, recovery of ova from the tube and the fertility of ova recovered were examined.
The results in June demonstrated that the superovulation was successfully induced by the preceding combined treatment with a satisfactory recovery and fertility of the ova ; no significant differences were observed between the oestrous and dioestrous group, except remarkable occurrence of the blood follicle in the former. In October and November, when the rabbit was in moulting, and the reproductive activity was to be lower, a little retardation in the response to the treatment in the number of both positive case and ovulation point was found. The pseudopregnant does(10th and 15th, when the treatment started, without progesterone administration), also indicated somewhat inferior results with the wider range of ovulation point number, and particularly, in 10 day, none; but in 15th day, moderate number of fertilized ova were obtained.
In conclusion, it can be said that the superovulation in rabbits is able to produce by PMS accompanied with progesterone pretreatment of which no work has ever adopted, though successful results hitherto were demonstrated only by the pituitary extracts or preparations. There are probably some differences according to the season of year in the response. The full data will be presented elsewhere adding the same kind of experiments carried on in March.

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緬羊の精液中のフラクトースおよびアスコルビン酸の測定をおこない次の結果を得た。
1.緬羊の精液中のフラクトースは季節的消長を示し,牝緬羊の繁殖期に相当する時期(9月~12月)に増加し,非繁殖期に相当する時期(3月~6月)に減少する傾向のあることを知つた。
2.精液中のフラクトースの季節的な増減から,緬羊の生殖器附属器官の機能に時期的な消長(周期)のあることが推察される。
3.夏季の高温多湿に原因するsummer sterilityの現象と精液のフラクトースの減少時とは時期的には一致しなかつた。
3.精液中のアスコルビン酸は1月から6月の間に増加する傾向がみられた。

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Further experiment of out-season of lamb production by PMS pretreated with progesterone was carried out with 23 ewes (two of them was lost by accidents) at the University Farm. Two groups of experimental consisted of PMS (Exp. I) and PMS followed with LH-rich gonadotrophin treatment (Exp. II). Four ewes with the same condition as to age and previous lambing results were allotted to one of the non-treated control (Cont. I). The other control (Cont. II) with another eight ewes untreated was also employed to refer the result obtained from the treatment. The reason of setting the non-treated control was due to the fact of a tendency of early lambing within the year. The mode and the name of hormones used were progesterone, Luteogen-Tomoda 12.5mg twice-daily for three days, followed 2 days intermission, and then PMS, Peamex-Tomoda 1, 000 i u. Exp. II was further administered with LH, rich gonadotrophin Synahorin-Teizo 200 Rb. u. on the next morning of PMS injection. During oestrus last from 2 to 4 days after about 48 hrs. of PMS treatment, ewes were properly served with two rams once a day. Before and after the treatment the ewes were put on the pasture. Taking pregancy period for 145±5 days, lambing date between 18 th and 28th November, was accounted for a positive. Results in lambing were as followed: Exp. I, 3/5, Exp. II, 2/4, Cont. I, 0/3, Cont. II, 0/5. Assuming no differences existed between I and II, experimental and control sum up to 5/9 and 0/8, respectively. So that it was again demonstrated the successive percentage of nearly a half. Negative cases proved fertility of ewes and some of them showed early lambing. As was reported in the previous report, oestrus was not so prominent that holding ewes by head for mating was needed, and was variable in its length. Since the authors had obtained some cases of failure in inducing ovulation (unpublished data, '59) LH-rich gonadotrophin was introduced to test the effectiveness in the lambing result, however, no advantage seemed to be expected so far as in the final result.
Further studies aiming improvement in the result and producing Autmn lambs are on progress.

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Interrelations between production and deposotion of yolk proteins in the liver and ovarian follicles of the laying hen were investigated through NaCl-Ac turbidity test and by marking the rapidly growing follicles in the ovary (RGF) with colloidal Sudan III.
Though there were marked individual differences from hen to hen, the concentration of yolk precur-sors in plasma, expressed by the degree of NaCl-Ac turbidity, increased with increasing ovarian activity except hen No. 8 (A, Fig. 3) in which elevation of.plasma yolk precursor occurred when index of ovulation decreased from 100 to 45%.
The results of time study indicated that the day of skip in egg laying was often predictable when RGF if suitable size was absent in the ovary.

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山羊の精液中のフラクトースおよびアスコルビン酸の測定をおこない次の結果を得た。
1.ザーネン種6頭中5頭の精液中のフラクトースは季節的消長を示し,牝山羊の繁殖期に相当する時期(10月~1月)に増加し,非繁殖期に相当する時期(5月~7月)に減少する傾向のあることが知られた。
2.精液中のフラクトースの季節的な増減は,牡山羊の生殖器附属器官の機能が時期的に消長周期を示すものと推察される。
3.日本在来種2頭とザーネン種1頭の精液中のフラクトースは季節的消長を示さず,年間を通してほぼ一定の量をたもつていた。
4.精液中のアスコルビン酸は例外はあつたが,7月から1月の間に減少し,2月から6月にかけて増加するもののようである。しかしこのような変化のみられない例もあつた。

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