ICSH及びHCGの単一皮下注射による成熟雌ラット及びマウスにおける排卵誘起について

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In general, the biological properties of ICSH and HCG are thought to be similar.However, some biological effects of HCG are distinct from that of ICSH. Differences in effects on the male reproductive organs were discussed in the preceding reports 6).
In the present paper, the potency of ICSH and HCG to induce ovulationwas compared. Adult female rats of the wistar strain showing the regular 4 dayestrous cycle were employed. Animals received the single subcutaneous injection on the afternoon of the day when the late diestrous smear stage wasshown. Twentyto twenty-two hours after the injection, they were sacrificed and the tubal ova were inspected under 10 time magnification. Hormones used were the same preparations as in the previous studies.6), 9) A given dose of the hormone was dissolved in 0.1 to 0.4 cc of saline on the day of the injection.
Summarized results of the induced ovulation by sheep ICSH and two preparations of HCG in rats areshown in Fig. 1. The dosage of gonadotrophins is expressed by the prostate unit, which is the convinient unit available to standardize the biological activity of any sort of gonadotrophic substances.6) To standardize the ovulation inducing activity of gonadotrophins, the authors define one rat ovulating unit as the amount ofhormone which will induce ovulation in 50 % of treated rats. One rat ovulating unit of preparations of sheep ICSH, purified and crude HCG is about 0.19, 11 and 25 Pr. U. respectively. Accordingly, it is clear that, in ICSH, the ovulation inducing potency is more active as compared with the effect stimulating androgensecretion, On the contrary, the ovulation inducing potency is very weak when compared with the androgen secreting activity in HCG. These results may give some suggestion in clinical apprication.
The dd strain mice is not so sensitive to sheep ICSH,