The effectiveness of HCG for treating ovarian disorders in cattle has been widely recognized. In our previous report, however, we demonstrated that the antihormone against HCG (Anti-HCG) was produced in considerable number of cattle which had been injected repeatedly with this hormone in order to treat ovarian follicle cysts and the majority of the Anti-HCG positive animals did not recover from this disease in spite of these treatments. Considering these facts, it appeared that the Anti-HCG positive sera might inhibit considerably gonadotrophic actions of HCG injected. In this report weperformed hormonal treatment of refractory ovarian follicle cysts in cattle by injecting HCG directly into the cysts. The purpose of the present studies was to ascertain whether this treatment was effective on cattle in which the Anti-HCG was produced. The animals used in the present studies were divided according to histories of HCG administrations into 2 groups, i. e. Group A including 10 animals and Group B including 12 animals.
Group A (shown in Table 1): The animals in this group had been received 1-3 injections of 10, 000-20, 000 MU or 5, 000-20, 000 IU of HCG intramuscularly within recent 4 months. They had not recovered from ovarian follicle cysts after these treatments and the serum Anti-HCG was determined to be positive in 6 animals (remaining 4 animals were not examined).
Group B (shown in Table 2): All animals in this group had never been received the HCG treatment.
The instrument used for injecting HCG solution directly into follicle cysts is shown in Figure 1. It was made up of a 10 cc. glass syringe and a vinyl tube (1.5 mm. in inside diameter and 55 cm. in length) attached to a needle (1.1 mm. in outside diameter and 4 cm. in length). The needle was introduced into the vagina with one hand, and thrust through the veginal wall. The cystic ovary was approched to the tip of the needle by the other hand per rectum. The needle was then stuck into the follicle cyst. After cystic fluid was drained off by sucking the syringe, 2-6 cc. of HCG solution was injected into the follicle cyst, The doses of HCG were decided according to histories of the HCG-treatments and conditions of the follicle cysts in the animals. The average dose injected into one sovery was 3, 540 MU (1, 000-6, 000 MU) in group A and 2, 100 MU (800-5, 000 MU) in group B.
The results obtained ware summarized as follows:
1. Group A: Four of 6 animals in which the serum Anti-HCG was determined to be positive, recovered respectively from ovarian follicle cysts by single treatment with HCG, and the other 2 animals recovered by double treatments. Three of 4 animals in which the Anti-HCG was not examined, recovered respectively by single treatment, and remaining 1 recovered by doble treatments.
Group B: Seven of 12 animals recovered respectively from ovarian follicle cysts by single treatment, and 2 of remaining 4 animals recovered by 2 or 3 treatments (one animal was sold after the 2nd-treament). Consequently, 9 of 12 animals (75.0%) recovered by this treatment.
2. In 19 animals in both groups which were recovered from ovarian cysts by this treament, days from the intrafollicular injection of HCG to the occurrence of normal estrus were 6-22 days (av. 14.0 days). It appeared that the periods from the HCG injection to recovery were relatively short in this treatment as compared with those in the customarily applied intramuscular or subcutaneous injections. Fifteen of these 19 cured animals were inseminated and 13 of them (86.7%) became pregnant.
3. From these results it was recognized that the injection of HCG solution directly into follicle cysts might be an available method of treating refractory ovarian cysts in cattle, especially in the Anti-HCG positive animals.
抄録全体を表示
