抄録
For a purpose of nonthrombogenic artificial heart, antithrombogenicity of Tetoron velour covered with fibroblasts of human embryo was studied. As the fibroblasts did not grow well and their viability tend to decrease after 48 hours incubation on the Tetoron velour, seeding of the fibroblasts (5×105/cm2) was added after 24 hours. After 48 hours incubation, Tetoron velour was inserted into the SVC of the mongrel dogs. After 2 hours exposure to blood stream, surface of neointima formed on the cell cultured Tetoron velour was thinner and smoother than that of the controls. Utilizing Helmholtz-Smoluchowski's formula, zeta potential of cell covered Tetoron velour was calculated, which revealed slightly positive. Adsorption of ion to cell membrane or bovine serum of culture medium were thought to be the cause of positive zeta potential. It is concluded that fibroblasts cultured on the Tetoron velour inhibits thrombus formation in early phase, though positively charged bioelectrically.
