日本細菌学雑誌
Online ISSN : 1882-4110
Print ISSN : 0021-4930
ISSN-L : 0021-4930
Mycobacterium smegmatis.その性状と同定法
束村 道雄水野 松司外山 春雄束村 純雄
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1966 年 21 巻 11 号 p. 666-671

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Physiological characters of twenty-four strains of Mycobacterium smegmatis, classified by a numerical classification, were investigated. The results are shown in tables 1 and 2.
Colonial morphology: White or creamy, wet and smooth; non-photochromogenic. Non-motile. Acidfast rods or short rods. Growth at 3 days on egg media and on Sauton agar. Growth at 28°C, 37°C and 45°C; no growth at 52°C. Growth at 0.1% picric acid and 0.2% picric acid in Sauton agar. Growth at 0.2% Na-p-aminosalicylate and at 0.1% Na-salicylate in Ogawa egg medium. Growth at 0.25mg/ml. 8-azaguanine in Ogawa egg medium. No growth at 0.5mg/ml. NH2OH. HCl in Ogawa egg medium. Niacin: Negative.
Nitrate reduction: Positive. Three-day-arylsulfatase: Negative. Two-week-arylsulfatase: Positive. PAS degradation: Negative. Salicylate degration: Negative. The following amidases are positive according to the meteod of Bönicke: Acetamidase, benzamidase, urease, isonicotinamidase, nicotinamidase, pyrazinamidase, succinamidase. The following amidase are negative in most strains: Salicylamidase, allantoinase and malonamidase.
All of acetate, citrate, succinate, malate, pyruvate, benzoate, malonate and fumarate are utilized as sole carbon source for growth. The following carbohydrates are utilized as sole carbon source for growth: Glycerol, glucose, fructose, sucrose, mannose, galactose, arabinose, xylose, rhamnose, trehalose, inositol, mannitol, sorbitol, ethanol, propanol, propylene glycel, 1, 3-butylene glycol and 2, 3-butylene glycol. Raffinose and 1, 4-butylene glycol not utilized as sole carbon source for growth.
Acid formed from glucose, mannose, galactose, arabinose, xylose, rhamnose, trehalose, inositol, mannitol and sorbitol, but no acid from raffinose.
All of the following compounds are utilized as sole nitrogen source for growth: L-Glutamate, L-serine, L-methionine, acetamide, benzamide, urea, pyrazinamide, nicotinamide, isonicotinamide, succinamide, nitrate and nitrite.
All of the following compounds are utilized as sole, simultaneous nitrogen and carbon source for growth: L-Glutamate, L-serine, glucosamine-HCl, acetamide, benzamide, monoethanolamine and trimethylene diamine.
Identification. The following characters are almost specific for M. smegmatis and are considered to be “distinguishing characters” (Table 3). (1) Benzamidase; (2) isonicotinamidase; (3) succinamidase; (4) acid from galactose: (5) acid from rhamnose; (6) acid from sorbitol; (7) utilization of benzoate as sole carbon source for growth; (8) utilization of benzamide as sole nitrogen source for growth; (9) utilization of benzamide as simultaneous nitrogen and carbon source for growth.

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