Abstract
This report described the several extraction methods (Saline, 90% phenol and hot water) and purification of mycelial antigenic substances of Aspergillus fumigatus.
The extraction methods are as follows:
In the first, myceliun of A. fumigatus homoginized with saline solution at 0°C, for 3hrs, and centrifuged with 3000r.p.m., and the residue extracted with 90% phenol, at 80°C, for 30 minutes, and then the phenol treated residue was extracted further with hot water at 100°C. for 30 minutes.
The composition of those fractions are as follows:
1) The carbohydrate contents are about 50% in those extraction methods. Sugar components are mannose and galactose from fractions by saline and 90% phenol extractions, but that of hot water extraction contain mannose, galactose and glucose.
2) Major components of fatty acid in those fractions are palmitic, stearic, oleic, linoleic and linolenic acids.
3) Those fractions showes almost similarity of antigenic titre.
