Breeding Science
Online ISSN : 1347-3735
Print ISSN : 1344-7610
ISSN-L : 1344-7610
Research papers
Identification of Asian Pear Varieties by SSR Analysis
Tetsuya KimuraYong Zhong ShiMoriyuki ShodaKazuo KotobukiNagao MatsutaTateki HayashiYoshiyuki BanToshiya Yamamoto
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2002 年 52 巻 2 号 p. 115-121

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Sixty Asian pear accessions from 6 Pyrus species were genetically identified by 9 SSR markers with a total of 133 putative alleles. Among them, 58 varieties could be successfully differentiated except for 2 pairs of synonymous or clonal varieties. All the SSR markers produced 1 or 2 discrete amplified fragments for all the diploid accessions, whereas a triploid variety showed 3 fragments with some SSRs. The number of putative alleles ranged from 7 to 20, with an average value of 14.8. The observed heterozygosity and the power of discrimination were 0.63 and 0.91, respectively. A phenogram based on the SSR genotypes was obtained, showing 3 major groups corresponding to the Japanese, Chinese and European groups. The SSR markers were highly polymorphic and could be utilized as a reliable tool for cultivar identification in Asian pears.

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© 2002 by JAPANESE SOCIETY OF BREEDING
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