Construction of a genetic linkage map of Lentinula edodes based on SSR, SRAP and TRAP markers

Hui Dong; Xiaodong Shang; Xiaoyan Zhao; Hailong Yu; Ning Jiang; Meiyan Zhang; Qi Tan; Changyan Zhou; Lujun Zhang

doi:10.1270/jsbbs.18123

Research Papers

Construction of a genetic linkage map of Lentinula edodes based on SSR, SRAP and TRAP markers

Hui Dong, Xiaodong Shang, Xiaoyan Zhao, Hailong Yu, Ning Jiang, Meiyan Zhang, Qi Tan, Changyan Zhou, Lujun Zhang

著者情報

Hui Dong
Institute of Agro-Food Quality Standard and Testing Technology, Shanghai Academy of Agricultural Sciences, Laboratory of Agro-Food Quality and Safety Risk Assessment at Shanghai
Xiaodong Shang
Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, National Research Center of Edible Fungi Biotechnology and Engineering
Xiaoyan Zhao
Institute of Agro-Food Quality Standard and Testing Technology, Shanghai Academy of Agricultural Sciences, Laboratory of Agro-Food Quality and Safety Risk Assessment at Shanghai
Hailong Yu
Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, National Research Center of Edible Fungi Biotechnology and Engineering
Ning Jiang
Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, National Research Center of Edible Fungi Biotechnology and Engineering
Meiyan Zhang
Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, National Research Center of Edible Fungi Biotechnology and Engineering
Qi Tan
Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, National Research Center of Edible Fungi Biotechnology and Engineering
Changyan Zhou
Institute of Agro-Food Quality Standard and Testing Technology, Shanghai Academy of Agricultural Sciences, Laboratory of Agro-Food Quality and Safety Risk Assessment at Shanghai
Lujun Zhang 責任著者
Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, National Research Center of Edible Fungi Biotechnology and Engineering

キーワード: Lentinula edodes, genetic linkage map, SSR

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電子付録

2019 年 69 巻 4 号 p. 585-591

DOI https://doi.org/10.1270/jsbbs.18123

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詳細

抄録

Genetic mapping is a basic tool for eukaryotic genomic research. It allows the localization of genes or quantitative trait loci (QTLs) and map-based cloning. In this study, we constructed a linkage map based on DNA samples from a commercial strain L808, including two parental monokaryons and 93 single spore isolates considered with segregating to 1:1:1:1 at four mating types (A₁B₁, A₁B₂, A₂B₁ and A₂B₂). Using Simple Sequence Repeats (SSR), Sequence Related Amplified Polymorphism (SRAP), Target Region Amplified Polymorphism (TRAP) molecular markers, 182 molecular markers and two mating factors were located on 11 linkage groups (LGs). The total length of the map was 948.083 centimorgan (cM), with an average marker interval distance of 4.817 cM. Only two gaps spanning more than 20 cM was observed. The probability of 20 cM, 10 cM, 5 cM genetic distance cover one marker was 99.68%, 94.36%, 76.43% in our genetic linkage map, respectively. This is the first linkage map of Lentinula edodes using SSR markers, which provides essential information for quantitative trait analyses and improvement of genome assembly.

責任著者(Corresponding author)

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