抄録
Peptides play crucial role in many physiological events. In the central nerve system, most neuron contains biologically active peptides together with conventional neurotransmitters. Neuropeptides to act as neurotransmitter, neurohormone or neuromodulator are involved in a wide variety of physiological processes including reproduction, growth, stress, sleep/wake cycles, feeding and many other pathways. Here, we performed the comprehenmsive peptidome analysis of mouse brain using 2D-microHPLC-MALDI-TOF-MS analytical platform developed by us. 2D-microHPLC-MALDI-TOF-MS is composed of ion-exchange (SCX) and reversed-phase (RP) high-performance liquid chromatography, followed by matrix-assisted laser desorption/ionization time of flight mass spectrometry. The peptides from mouse brain were separated into 6 fractions by SCX and then each fractions of SCX were separated into 190 fractions by RP (total 1140 fractions), and each fraction is analyzed with mass spectrometer. More than 4500 ion peaks were observed with m/z from 700 to 7000. These MS data was processed using gel viewer as two-dimensional virtual peptide map where the x-axis displays m/z and the y-axis displays fraction number of RP. MS/MS sequencing and database tools identified over 1000 different peptides including several known neropeptides and its precurcer proteins.
