抄録
Heavy metal wastewaters (Wastewater A: inorganic waste containing heavy metals as well as ferrous sulfate and alkaline after treatment for removal of mercury, fluoride, phosphate and cyanide, Wastewater B: additional treatment by ferrite formation, activated carbon and alumina added to wastewater A) were analyzed using a yeast DNA microarray to elucidate the toxic mode of action. Differences were observed in both the number of genes induced and Munich Information Center for Protein sequence (MIPS) functional categories between these heavy metal waste samples. The expression of a number of genes changed in response to exposure to heavy metal wastewater A. After treatment of heavy metal wastewater A with ferrite formation, active carbon and alumina, wastewater B decreased the number of induced genes in every category except that concerned with regulation and interaction with the cellular environment, while the number of repressed genes decreased in every category. These results suggest that a DNA microarray can be a powerful tool for bio-monitoring the fate of heavy metals in the environment, and for evaluating the effectiveness of water treatment systems in reducing heavy metals.
