A Novel, Selective, Ready-to-Use Culture Medium for the Enumeration of Bacillus cereus

抄録

Bacillus cereus is a Gram-positive, sporulating microorganism that causes food poisoning and / or spoilage. Hence, it is important to detect and enumerate B. cereus; this typically involves culturing with mannitol egg yolk polymyxin agar and NaCl glycine Kim Goepfert agar, both of which require the addition of aseptic egg yolk. To eliminate this unstable and variable step, we developed and evaluated a novel B. cereus group-specific ready-to-use dry medium, CompactDry BC. Inclusivity study results revealed that CompactDry BC showed growth comparable to classical media. Exclusivity study results revealed no typical blue colonies on CompactDry BC. Relative trueness study results revealed that CompactDry BC showed a linear association and almost no negative bias compared to the classical media. Furthermore, we also report the comparison studies of CompactDry BC with CompactDry X-BC, which detects the B. cereus group. Throughout the experiments, CompactDry BC showed higher growth of the B. cereus group than CompactDry X-BC.