抄録
A colourimetric DNA hybridization method (DNA probe method) was applied for detection of Campylobacter jejuni/coli in poultry specimens. A total of 32 specimens of chicken meats obtained at 6 retail stores (collection 1) was supplied for isolation of C. Jejuni/coli by conventional culture. The organism was isolated from 3 specimens by the direct smeared agar method (Skirrow's and Butzler's agars supplemented with the agents, Oxoid SR 69 and 85), and from 21 specimens with culture of 3 enrichment broths containing each supplement of Skirrow, Butzler, and Preston (Oxoid SR 69, 85, and 117). The surface of the direct smeared agar was covered with bacteria other than C. jejuni/coli after incubation. When the DNA probe method was applied for suspension of bacterial harvests from the agar surface, C. jejuni/coli was detected in 10 out of 32 specimens. However, the organism was not detected in any broths by the DNA probe method applied for the enrichment cultures. As a case of the specimens from which C. jejuni/coli was easy to be isolated because of low contamination with bacteria which disturb the isolation of C. Jejuni/coli, among 11 specimens from 2 stores (collection 2) and 5 specimens from 1 store (collection 3), C. jejuni/coli was isolated from the direct smeared agars of 7 and 4 specimens, and detected by the DNA probe method in 6 and 4 specimens, respectively. Furthemore, from the Preston enrichment broth, the isolation rate of the organism was 8/11 (collection 2) and 4/5 (collection 3), and the detection rate by the DNA probe method was 4/11 (collection 2) and 1/5 (collection 3). When the Preston broth including the specimens of collection 2 was inoculated with 104 colony forming units /ml of C. jejuni, the organism was detected from all the enrichment cultures by both the culture and the DNA probe metods.
