抄録
A synthetic medium for the production of trichothecene mycotoxins by Fusarium species has been developed. Fusarium sporotrichioides F-135 strain was inoculated in a synthetic medium (SSA) containing sucrose, salts, asparagine and Ca-panthothenate. In 14 days of still incubation at 25°C, the organisms produced 2, 265μg/ml of T-2 toxin and 420μg/ml of diacetoxyscirpenol. The medium also supported production of a large amount of fusarenone X, nivalenol, neosolaniol, butenolide and moniformin by the organisms. The results indicate that the SSA medium appears to be suitable for production of trichothecens and other mycotoxins by Fusarium species.
The medium consists of the following components: sucrose 200g, Asparagine 10g, Capanthothenate 0.01g, NaNO3 1g, MgSO4·7H2O 1g, KH2PO4 0.75g, ZnSO4·7H2O 0.1g, FeSO4·7H2O 0.01g, MnCl2·4H2O 0.001g, CuSO4·5H2O 0.001g, Co (NO3) 2·6H2O 0.0001g, NaMoO4·2H2O 0.001g, NiCl2·6H2O 0.001g, Na2B4O7 0.001g and distilled water 1, 000ml.
