食品と微生物
Online ISSN : 1884-5754
Print ISSN : 0910-8637
ISSN-L : 0910-8637
ブドウ球菌の増殖とエンテロトキシン産生に及ぼす食品添加物の影響について
郷原 秀一寺田 厚内田 和夫鏡 晃
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ジャーナル フリー

1988 年 5 巻 2 号 p. 121-129

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Potassium sorbate (PS), sodium nitrate (SN), condensed phosphate, sodium chloride, and glycine, added singly or in combinations, to Brain Heart Infusion (BHI) at different pH values were examined for the inhibitory effects on growth and enterotoxin A production of Staphylococcus aureus (S. aureus) in inoculum sizes of 103 and 106 cells/ml and at 35°C. Only a slight inhibition of S. aureus was observed at pH 5.0 and aw0.99, and it decreased as pH was raised to 6.0 and 7.0.
Some inhibitory effects of PS were seen at 1, 000 ppm and 2, 000 ppm inoculated with 103 and 106 cells/ml of S. aureus at pH 5.0, respectively and 20, 000 ppm at pH 6.0 and 40, 000 ppm at pH 7.0 and aw 0.97 on the both inocula.
Growth and enterotoxin production of S. aureus were inhibited by SN of 75 ppm with the inoculum of either size at pH 5.0, but was not controlled by the addition of 750 ppm SN at pH 6.0.
Sodium acid pyrophosphate (SAPP) at 4, 000 ppm was not effective on S. aureus at any pH, but sodium ultrapolyphosphate (SUPP) and sodium metaphosphate (SMP) at 4, 000 ppm were very effective on the both inocula at pH 7.0. Sodium tripolyphosphate (STPP) and a 2: 1 mixture of STPP and SAPP (TPAP) at 4, 000 ppm inhibited growth and enterotoxin production of S. aureus on the inoculum of either size at pH 5.0, and on the inoculum of 103 cells/ml at pH 6.0, respectively. STPP at 2, 000 ppm was effective on the inoculum of 103 cells/ml of S. aureus.
The combination of 200 ppm of PS and 35 ppm of SN at pH 5.0 and 2, 000 ppm of PS and 35 ppm of SN at pH 5.5 exhibited a synergistic activity on the both inoculums of S. aureus.
The combination of 75 ppm of SN and 2, 000 ppm or 1, 000 ppm of TPAP at pH 6.0 also showed clear inhibition on the both inocula and on the inoculum of 103cells/ml of S. aureus, respectively. At pH 6.5, 75 ppm of SN and 4, 000 ppm of TPAP showed a effective inhibition on the inoculum of either size.
The combination of three agents, 2, 000 ppm of TPAP, 75 ppm of SN, and 2, 000 ppm of PS showed a high effect more rapidly in about 12 hours than without PS at pH 6 .0 on S. aureus. When NaCl was further supplemented, growth was slightly inhibited, especially by the addition of 3.5% NaCl that has got up speed about 8 and 16 hours when inoculated with 103 and 106 cells/ml of S. aureus, respectively.
At pH 6.5 and aw 0.95, growth and enterotoxin production of S. aureus in BHI inoculated with 103cells/ml were completely inhibited by the combination of 3, 000 ppm of TPAP, 75 ppm of SN, 1, 000 ppm of PS, 3.5% of NaCl and 1% of glycine after 72 hours of incubation.

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