1989 年 6 巻 2 号 p. 103-106
A total of 171 soil samples were collected from coasts, inlets, ponds, marshes and artificial pond in Miyazaki Prefecture to survey for Clostridium botulinum from April, 1987 to September, 1988. Botulinum toxin was detected in enrichment cultures of 18 (10.5%) out of 171 soil samples. These toxins were identified by the neutralization technique; two were type C2 and the other 16 were neutralized equally with either monovalent anti-toxin type C1 or D.