抄録
The new photosensitizer, PH-1126 (Hamari Chemical Co. Ltd. Osaka) has amine in the d-ring of pheophorbide. The agent is considered to be more useful for PDT due to its greater absorption band in longer wavelength region. In order to investigate its efficacy, tumor-bearing rats were sacrified at intervals from 2 to 96hours after PH-1126 administration of 10mg/kg intravenously. Samples of each organ were frozened, sectioned to 30μm thickness, and the fluorescence value was measured with excimer dye laser endoscopical fluorescence spectrophotometer using 405nm excitation beam. Also, the tissue concentration was analyzed by means of the high performance liquid chromatography.
The tissue concentration among organs such as lung, heart liver, spleen, kidney and skin kept high level till 24hours after administration, and then quickly decreased after 48hours.
Maximal PH-1126 level in brain tumor was 3.2μg/g at 24hours after administration. The other normal brain tissue such as cortex and white matter showed no presence of fluorescence, morover PH-1126 level in normal brain tissue were scant, 0.05μg/g. The ratio of tumor and normal brain tissue concentrations of PH-1126 was 64 to 1.
The PH-1126 uptake or retention into brain tumor was greater than that into normal brain tissue.
