抄録
In the present work, radiobiological studies on adrenoehromemonosemicarbazone placing special emphasis on its protective effects were conducted. The following results were thus obtained.
1. The local application of AC-17 failed to prevent skin damage of rabbit caused by the daily irrakiation of 300 r. With the intramuscular injections of 0.1 mg/kg and 1 mg/kg, 9, 200 r and 10, 000 r respectively were permissible against the chntrol value of 8, 000 r when judged by the skin erosion.
2. In the control cases, complete recovery from the skin erosion was not noted even after 1 year, but with the intramuscular injections only 2 months were required for complete recovery.
3. In the group received 1 mg/kg intramuscular injections, it was histologically noted that the damage, especially the damage in the blood vessels, was found slight even when irradiated 9, 000 r for 30 days.
4. The effect of AC-17 against the fatal damage in the mice caused by total irradiation with 700 r (L. D. 100) was observed. The most favorable rate of survival; (41%) was obtained with one administration of 2 mg/kg before irradiation. Similar application of 10 mg/kg demonstrated almost no effect. When administered after irradiation, 25% was the highest rate of survival with 2 rng/kg in one dose. The rate was 5.5% with 4 mg/kg and 0% with 10 mg/kg showing no effect with larger doses.
5. The rate of survival was 30% when administered for 10 consecutive days before irradiation, with the consecutive administrations after irradiation, the rate was only 16% at the dose of 0.2 mg/kg, but all animals died at any other doses.
6. Especially when 2 mg/kg was administered before irradiation, diarrhen was found mild and the decrease in the body weight was only 1.3 g on 8th day.
In the rats administered similarly with 2 mg/kg, the decrease in the weight of spleen was milder compared with the control. The adrenal gland was found even hypertrophic compared with the control.
7. A total of 18, 000 r was irradiated to rabbit and the hematological changes were traced for 9 months. The results demonstrated that there seemed no difference in the decrease in WBC, RBC and Hb value between those received daily administration of 1 mg/kg of AC-17 until the completion of the irradiation and the control.
8. The combined effect of AC-17 and X-ray (700 r locally applied) on the Ehrlich's subcutaneous tumor wat investigated. Either in the local administration or in the general application of AC-17. at least, its sensitiveness to radiation was not diminiched.
9. In view of the above, the protective effect of AC-17 against radiation damage was considered to be due to its effects on blood vessels as well as greatly to its stimulating action on adrenal gland.
