抄録
In this study, the simulated transplantation experiment is used mouse iPS (induced pluripotent stem) cells. Mouse iPS cells are differentiated into neurons by SFEBq method. The mouse iPS cells are induced to differentiate into neural progenitor cells by suspension culture for 5 days, and then differentiate into neuron by adhesion culture for 5 days. Then, 2 cells are prepared, recipient cell and donor cell. First, suspension culture of recipient cell is started. Then, 5 days later, suspension culture of donor cell is started with recipient cell shifted adhesion culture. In 5 more days later, simulated transplantation of donor cell by adhesion culture is started next to recipient cell. The neuron networks between recipient cell and donor cell are jointed for another 5 days culturing. Then, the dynamic stimulation is applied to the neuronal networks to promote formation and merging. The 3-D vibration stage is used for imposing dynamic stimulation. The total stimulation time is 24 and 48 hours, the vibratory stimulations are applied with interval of once per 12 hours during 5 days. As a result, it is suggested that the longer total stimulation time is applied to, the more neuron networks are constructed.
