超音波振動子と光ファイバ型音圧プローブによる細胞接着力測定

抄録

Cell adhesion is critically important for a variety of cellular properties. However, an effective method to measure cell adhesion has not been reported yet. Here, we have designed a detachment method to detach cells by ultrasonic irradiation. To observe small amounts of cells, an ultrasonic irradiation device, which allow cell culturing in a PDMS chamber, was fabricated. In addition, an acoustic pressure probe of fiber optic type was fabricated to measure the acoustic pressure of the ultrasonic irradiated to the cells. With these devices, the acoustic pressure in the PDMS chamber was confirmed to be proportional to the applied current. Furthermore, by increasing the applied current of the piezoelectric transducer, the cells began to detach due to the ultrasonic irradiation was observed. That is, acoustic pressure of the cells in the PDMS chamber at the start of cell detachment was measured. These results provide additional insights into the physical characteristics of cell adhesion.