Development and Performance Evaluation of Dilution System for Diagnosis Systems with Saliva

抄録

In order to use saliva for human stress diagnosis, ELISA (Enzyme-Linked ImmunoSorbent Assay) is most promising, when saliva must be diluted in purified water from about 10¹ to 10⁴ times corresponding to the applied stress markers. A new dilution system with 4 diluting ladders in which each step has 10 times dilutive capability is developed, hence, can realize 10¹ to 10⁴ step-likely. Its performance was tested by having mixed a fluorescent material in pure water and checked its change of intensity, and it is verified that +-20% error for the 10⁴ time dilution target is kept.