スラグ内部循環流によるペプチドのがん細胞スフェロイドへの付着効率の向上

抄録

In order to find applicable target molecules for molecular target anti-cancer drugs, molecules on the surface of cancer cells in a peptide screening process should be close to those in a human body. A cancer spheroid is a cancer cell aggregate and shows properties similar to tissues. We previously proposed and achieved a peptide screening method using floating spheroids which stirs peptides and spheroids in a liquid slug using an internal convection to promote the peptide adhesion onto the spheroids. In this study, we investigate adhesion efficiency dependent on the speed of the liquid slug in the range from 7.2 to 31 mm/s. At a low speed (7.2 mm/s), antibodies as a simulation of a peptide do not adhere on the spheroids due to weak internal convection in the liquid slug. At a high speed (31 mm/s), the antibodies hardly adhered on the spheroids. We found the speed around 20 mm/s showed the efficient adhesion.