自動スフェロイド培養装置によるスフェロイドサイズの調整

抄録

This research group has established an original technique to build three-dimensional Cells Construct in Regenerative Medicine without using Scaffold. Cells construct is made by the cell that was cultured spheroid. The quality of spheroids is important to make the assumed cell construct. Now, the quality of the spheroid depends on the skill of technical experts, and a lot of fluctuation factors exist, so it is required to eliminate fluctuation factors to minimum. Therefore, this research was aimed to develop automatic spheroid culture System and adjust the spheroid size. We used mesenchymal stem cell of rabbit for experiment. Observation of spheroid was conducted every 24 hours for 5 days. Results of comparing spheroids made by the developed system and technical experts, we were able to build spheroids with smaller diameter variations and higher circularity than technical experts.