in-vivo再生治療を目指した接着細胞への遺伝子導入の研究

抄録

Direct reprogramming, which is a technology inducting particular kind of cells from other somatic cells, gains a lot of attention as a new method for regenerative therapy. A main process of the direct reprogramming is transfer of gene and/or low-molecular-weight compounds, and the process has difficulties when the technology is applied to in-vivo environment. Viral vectors are often used in laboratory scale but the safety concern of them caused by stochastic nature cannot be ignored. Therefore non-viral gene transfer is required and a number of physical methods are proposed but they often have disadvantages such as low efficiency and high invasiveness. In this study, we focused on the impact pressure generated by the collapse of the electrical-induced bubbles which can be applied simply. This pressure can work as local and minimally invasive mechanical stimuli for gene transfer from the outside of the affected part. For this study, we have achieved recognizing permeabilizing of cell membrane and transfer of GFP plasmids to NIH 3T3.