TAGレンズとイメージインテンシファイアを用いた蛍光ビーズの高速三次元トラッキング

抄録

In the medical and biological fields, three-dimensional measurement in a minute space is required, especially when the observation object is a moving object, and it is necessary to track the object at high speed. In addition, fluorescence microscope used in biological observation is an important technique for observing living organisms, as it enables limited illumination and observation of specific areas to be observed. In this study, we developed a prototype system for high-speed 3D tracking in fluorescence observation by combining a TAG lens, which is a high-speed focus tunable lens, and an image intensifier, and achieved millisecond-order 3D tracking of fluorescent beads.