抄録
The growth promoting activity of a little dose of TATD on young small rats and curative effect of the minimum amount of TATD on thiamine deficient rats were examined. And it may well be said that the biological thiamine activity of the minimum amount of TATD is found to be equal to that of equi-molecular amount of thiamine hydrochloride.
When a large amount of TATD was given to the rats orally, predominant and continuo us increase of thiamine contents of principal organs and a high urinary excretion ratio were observed. It was assured that absorption rate of TATD from intestine was excellently higher than that of common thiamine hydrochloride.
Incubating 35S (lipoic acid labelled)-TATD with heparinized human blood and washing with human plasm, thiamine content and specific radioactivity of blood cells and the rinsings were estimated. As the results, it was supposed that thiamine part of TATD was concentrated and remained in blood cells and lipoic acid derivative part was rapidly reprojected into plasm.
When a large amount of 35S-TATD was injected intravenously to the rats, increases of thiamine content and specific radioactivity of several organs were examined. And it was ascertained that TATD devided into thiamine part and lipoic acid derivative part promptly, and thiamine part had a great affinity for liver, kidney and heart, and lipoic acid derivative part had it for liver and kidney, and both parts acted separately and characteristically in the organism, and lipoic acid derivative part was excreted more rapidly than the other part into the urine.
