Abstract
Establishment of the micro-analytical method of polyoxyethylene fatty alcohol ether (SAEO) in peeled Mandarin orange was done.
To a homogenate of peeled orange, twice volumes of ethyl alcohol were added and the precipitate was centrifuged off. The supernatant was treated four times with ethyl acetate-chloroform (1: 1) mix-ture, and the extract was concentrated in vacuo. The residual concentrate was chromatographed by TLC method using silica gel G.
By this method, it was found that the minimum detectable concentration of SAEO in orange was 2ppm, and it became clear that there was no residual SAEO detectable in the samples which were prep-ared with SAEO seaking method.
