When anaerobic glycolysis of cancer tissue was studied by Warburg manometer, glucose was readily metabolized but galactose was hardly metabolized. As a fundamental study of the possibility of diet therapy of cancer by galactose, the problem “How matured rats utilize galactose ” was investigated.
When rats were fed by galactose as carbohydrate in diet, cataract was produced, urine volume increased, a large amount of galactose was excreted, body weight did not increase and rats were dead in about 20 days. Namely, matured rats can not utilize a large amount of galactose.
Thus, galactose-1-phosphate uridyl transferase activity in the liver, which plays important role in galactose metabolism, was determined with development of rat.
The activity was high in the stage of suckling but after deprivation of weaning (20 days after birth), the activity was decreased to the level of matured rats. On the contrary, the activity of glucokinase was low in the stage of suckling, and increased rapidly after weaning. The activity of hexokinase did not show any remarkable change with development of rats, and the activity was always low. The activities of these enzymes, which were concerned with carbohydrate metabolism, showed similar pattern in guinea-pigs which have different weaning (5 days after birth).
When the experimented animals just before weaning were fed by diet with galactose, the decrease of galactose-1-phosphate uridyl transferase could not be protected.
Cortisone, the adrenocortical hormone, inhibited galactose-1-phosphate uridyl transferase activity and promoted glucokinase activity.
When galactose tolerance was investigated on adult persons, less than 0.3g of galactose per kg body weight was utilized.

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The distribution of total and free amino acids in the TCA soluble fraction was studied in the course of natto manufacture, by means of paper-and ion-exchange (resin) column chromatography, following the previous report.
1) Nineteen kinds of amino acid including β-Alanine and an unknown spot were confirmed.
2) The TCA soluble total amino acid markedly increased in each step of processing, and the amino acid content of peptide form increased more significantly than that free amino acid, in which Lys, Asp and Glu contents were high. In the commercial natto products the similiar trend was indicated.
3) During natto processing the ratios of amino acid liberation (ratio of free amino acid present to total amino acid) gradually decreased, and in commercial natto products the value was higher than the others. The ratios of liberation with each amino acid were markedly low with Cys, high in Try and Tyr in all the samples tested.
4) The Asp and Glu contents in the total amino acids and peptides as well as the ratios of Asp and Glu liberation suggested that in the commercial strains for natto manufacture the Asp liberation capability is apparently greater that for Glu, and in Bacillus natto No. 6 vice versa.

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The effect of polyoxyethylene fatty alcohol ether (SAEO), a hydrochloric acid soluble nonionic surface active-agent, was investigated about its application on the peeling process of Mandarin orange. The experiments were carried out either laboratory-scale or a lot-process scale.
By the addition of SAEO to hydrochloric acid solution, it was found to be possible to lower the hydrochloric acid and sodium hydroxide concentrations necessary for the peeling process.
It was also observed that the soaking orange in SAEO aquous solution as the pre-treatment made the peeling process effective.

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Establishment of the micro-analytical method of polyoxyethylene fatty alcohol ether (SAEO) in peeled Mandarin orange was done.
To a homogenate of peeled orange, twice volumes of ethyl alcohol were added and the precipitate was centrifuged off. The supernatant was treated four times with ethyl acetate-chloroform (1: 1) mix-ture, and the extract was concentrated in vacuo. The residual concentrate was chromatographed by TLC method using silica gel G.
By this method, it was found that the minimum detectable concentration of SAEO in orange was 2ppm, and it became clear that there was no residual SAEO detectable in the samples which were prep-ared with SAEO seaking method.

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The present report describes experiments designed to derive the effect of tangle on the blood pressure and urinary excretion of sodium in rats.
Basal diet was consisted of mostly milled washed rice, solvent extracted soybean, thiamine and vitamin A.
The diet was made high salt diet by inclusion of 2.5g sodium chloride per 100g of diet. Powdered tangle, its ignited residue, its water extractive matter or extracted residue were included in the basal diet. Four feeding experiments were conducted in all. In every case the lower blood pressure was observed in rats fed with tangle supplemented diet than in those fed the basal diet, while the water extractive matter and the ash of tangle has little effect if any on the blood pressure.
The excretion of urinary sodium has increased in rats fed the diet after the addition of tangle or water extracted residue to the basal diet.
Since the amount of riboflavin and pantothenic acid was found to increase in liver, kidneys and feces of rats fed tangle or water extracted residue, present authors speculated the increased supply of riboflavin and pantothenic acid by the intestinal synthesis may favour the excretion of urinary sodium and prevent the elevation of blood pressure induced by high salt diet.
Koyanagi et al. have reported the increased excretion of sodium in urine and prevention of high blood pressure by supplementing riboflavin and pantothenic acid to the high salt diet consisting of rice and defatted soybean flour^{12) 13)}.

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Wistar strain rats were fed on a diet usually taken by babies in the Tohoku (northeastern) district of Japan and the effect of Sumithion, an agricultural insecticide, in the diet was examined. Increase in the amount of eggs or addition of beef liver to this diet prevented inhibition of rats growth by Sumithion to a certain extent. It was also found that the content of ³²P, used as a tracer, increased in various tissues of these rats and the content of inorganic phosphorus in the liver also increased at the same time.

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The physique, growth of height and weight in a year and intakes of energy and protein were studied in children of 6 to 14 years old from a primary school (75 boys and 103 girls) and a secondary school (37 boys and 54 girls) in Koto-ku, Tokyo. The intake of nutrients was estimated by the results of a three-day survey on their diets in June, 1967 and 1968 for the primary and secondary schools, respectively.
Though their growth in a year did not differ from the values calculated on the national averages, their physique; height and weight, deviated diversely from the national and Tokyo average values. The values of allowance in nutritional intake; energy and protein, which was recommended by the Ministry of Health in 1969, were modified with the actual physique of children studied and also with retraction of safety factor; 10% in the allowance of energy and 70% in the protein.
The children of the primary school had the same level of energy intake to the modified allowance by the physique and the retraction of safety factor, and their protein intake had the excess of 30 to 90% to the modified allowance.
In the secondary school students, their energy intake was short of the modified allowance and the protein intake was in accord with it.

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Sphingomyelin was purely isolated from egg yolk lipids and examined for composition of the fatty acid constituents and the long chain base ones. As the component eleven fatty acids were detected. Among them, palmitic acid was predominant and lignoceric acid was in the second place. As long chain bases were recognized sphingosine and dihydorsphingosine. The former was found to be in a greater part.

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Forty-four girls in a certain secondary school in Koto-ku, Tokyo, were studied in terms of their age at menarche, growth, physique and intake of energy and protein.
1) The median of the age at menarche was 12 years 10 months and the range; 11 years-14 years 5 months.
2) The age from menarche correlated significantly positively with the height, weight and Rohrer index, and negatively with the growth of height from 1967 to 1968, Ponderal index, energy intake per body surface and protein intake per body weight.
3) The amount of decrease in a year after menarche was 2.85 Cal/m²/hr in the energy intake per body surface. This value corresponded to the reported decrease in the basal metabolic rate after menarche.
4) The differences of growth rates in weight and body surface and of change in the basal metabolic rate used as the standard for National Allowance of Nutritional Intake from those obtained in this survey, were discussed as probable causes in making a considerably large gap between the Allowance and the actual intake.

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General analysis of foods, analysis of tyramin and histamine were carried out on nineteen samples of the Korean miso. Results indicated that the ratio of the soluble nitrogen or the amino nitrogen to the total nitrogen were directly proportional to the moisture contents respectively, and the ratio of the soluble nitrogen to the amino nitrogen also was shown the same tendency. The pH values were neutral or slightly acid side in the outer portions of the miso balls, although there were neutral or slightly basic side in the inner portions. The growth of mold were controled by the moisture level in samples, and the outer portions that were rich in water were overgrown with mold. Tyramin and histamine slightly existed, however these almost could be ignored, except for two samples.

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