抄録
Serine acetyltransferase (SATase) catalyzes the formation of O-acetylserine (OAS) from L-serine and acetyl-CoA, connecting serine metabolism to cysteine biosynthesis. SATase and OAS are proposed to be regulatory factors in the biosynthesis of cysteine in plants. In the genome of Arabidopsis thaliana, there are five predicted SATase-like genes. To elucidate the temporal and spatial expression patterns of the different putative SATase isoforms (SAT-p, -c, -m, -#5, and -106) during the development of wild type A. thaliana, real-time quantitative PCR was performed. The results show that SATases mRNAs have distinct expression levels between aerial and root regions during plant development.
A. thaliana plants carrying fusions of SATase promoter region and green fluorescent protein (GFP) were produced to verify SAT-m, -c, and -p tissue-specific expressions. The result showed similar tissue-specific expressions of SAT-p and SAT-c, differing with SAT-m that is expressed ubiquitously.
