抄録
A cDNA microarray was utilized to screen for clones that are induced by chilling stress in rice roots. Two cDNAs clones induced at 12oC and involved in trehalose biosynthesis, TPS and TPP, were identified. Full-length cDNA clones were isolated through library screening. Southern analysis indicates, OsTPP1 is a single copy gene while OsTPS1 may have similar genes. Northern blotting showed that both clones were rapidly and transiently induced within 1-4h and the signals disappeared after 6-10h of chilling stress. Fructose and glucose levels in cold-treated rice roots consistently increased after a lag of 5h. Sucrose levels consistently increased during the 24h of chilling treatment. Concurrent increase of trehalose levels was apparently faster and earlier than the other sugars, and followed the patterns of OsTPP and OsTPS mRNAs accumulation. It appears that trehalose biosynthesis is transiently induced and maybe involve in the regulation of sugar metabolism in chilling-stressed roots in rice.
