抄録
With the aim of understanding the whole genetic system of legume species, we initiated large-scale sequencing of the genome of a model legume, Lotus japonicus. Using the information on ESTs and cDNA markers of L. japonicus and other legumes, genomic clones corresponding to the multiple seed points of the genome have been chosen as initial targets. As the accumulation of the seed sequences has progressed, clone selection by walking has also been initiated. As of November 2002, a total of 1130 clones have been selected. Eighty-four of them are in the library phase, 6 are being sequenced, 570 are in the finishing phase, 68 are being annotated, and 237 have been annotated. Mapping of the seed clones has also been performed by generating PCR-based markers from sequence information, and 502 clones have been genetically localized. The detailed information on this project is available through the web site at http://www.kazusa.or.jp/lotus/.
