抄録
Functional aberrations in isolated mitochondria and regulation of AOX1 gene were investigated in tobacco cells (Nicotiana tabacum L.,nonchlorophyllic) under aluminium (Al) stress. Respiratory functions and ATP synthesis showed a greater inhibition in mitochondria isolated from SL (Al-sensitive) cells as compared to ALT301 (Al-tolerant) cells after 18h of Al treatment. A decrease in AOX-pathway and CYT-pathway capacities was seen in SL cells under Al stress. Whereas, 15 % increase in AOX-pathway capacity was observed in ALT301 cells mitochondria. An increase in mitochondrial ROS was observed in SL cells upon Al treatment. But a seemingly decrease in mitochondrial ROS production was observed in ALT301cells. Al stress downregulated the expression of AOX1 gene in both cell lines. However, Aox protein level confirmed through western blot remained high in ALT301 under Al stress as compared to SL suggesting a post-translational modification that might confer Al tolerance traits.
