抄録
Cytokinin N6-benzyladenine (BA) induces rapid apoptotic cell death in tobacco BY-2 cells. We show that this cell death is independent of the presence or absence of auxin (2,4D) in the cultivation media and can be partially suppressed by protein synthesis inhibitor cycloheximide. Nitric oxide (NO) plays important role in stress signal transduction. We noticed that NO donors sodium nitroprusside (SNP) and S-nitroso-N-acetyl-D,L-penicillamine (SNAP) induce cell death in similar manner as BA in cultured BY-2 cells. We further show that the NO scavenger carboxy-PTIO can reduce both NO and BA-induced cell death process in concentration-dependent manner.
We used the 16K BY-2 tobacco microarray to compare the response of cells to both cell death-inducing factors at gene expression level. The presence of commonly induced and suppressed genes by NO and BA further suggests that nitric oxide may possibly play an intermediate role in the process of cell death induced by cytokinin application.
