抄録
Jasmonates represent a useful tool in the investigation and engineering of natural bioproduction (secondary metabolism) in plants. In tobacco bright yellow-2 (BY-2) cells, treatment with methyl jasmonate (MJ) induces the rapid accumulation of alkaloids and various phenylpropanoids. We employed a comprehensive cDNA tobacco microarray to isolate novel signal transduction component, connecting the MJ-hormone signal to secondary metabolism-related gene expression. By overexpression analysis, we show that the tobacco MJ-inducible MYB-family transcription factor NtMYBJS1 positively regulates transcription of several early phenylpropanoid-related genes, causing accumulation of feruloylputrescine and caffeoylputrescine in cultured cells. These results are further supported by the gel mobility shift assay results, showing that this MYB gene can selectively bind to specific DNA motives in the tobacco PAL gene promoters. In conclusion, we propose that the NtMYBJS1 protein functions in tobacco MJ signal transduction, inducing phenylpropanoid biosynthetic genes and the accumulation of phenylpropanoid-polyamine conjugates during wound and/or pathogen stress.
