抄録
We have applied activation tagging with enhancer sequences derived from cauliflower mosaic virus to selection of mutants tolerant to salt in fundamental cell functions, and further identified causal genes. Out of 62,000 transformed calli screened, more than 25 mutants were identified to be resistant to 150 mM NaCl. Thermal asymmetric interlaced PCR (TAIL-PCR) was carried out to determine the locations of T-DNA integration in the genome. Expression of the genes adjacent to the T-DNA insert was analyzed at normal (no NaCl) and 150 mM NaCl-containing media through real-time PCR by LightCycler. In one of the mutants, expression of a gene (patent to be applied) was found to be enhanced more than 10 times at the normal medium while more than 100 times at 150 mM-NaCl stress level. Regenerated mutant plants showed enhanced tolerance to salt stress as compared to the wild-type. The expression profiles are being analyzed for other mutants.
