抄録
We previously demonstrated interactions among peroxisomal biogenesis factors, Pex5p, Pex7p and Pex14p, peroxisomal proteins with two targeting signals, PTS1 and PTS2, in Arabidopsis thaliana using yeast two-hybrid analysis. In the present study, we introduced bimolecular fluorescence complementation (BiFC) assay using split yellow fluorescent protein (YFP) to visualize the interactions between these proteins in living plant cells. When nYFP-PTS1/PTS2-cYFP or nYFP-Pex7p/PTS2-cYFP fusion proteins were expressed transiently, onion epidermal cells showed punctate green fluorescent signals that were co-localized with tdTomato-PTS1. The result indicates that PTS1-PTS2 and Pex7p-PTS2 interacted with each other in vivo in the peroxisomes On the other hand, when cYFP-Pex5p and nYFP-Pex7p were expressed together, onion epidermal cells showed cytosolic green fluorescent signal, suggesting that Pex5p and Pex7p interacted with each other in the cytosol. In this report, we propose a revised model for the import of PTS-containing matrix proteins.
