抄録
B-type cyclins are known as mitotic cyclins. We have isolated and sequenced a cDNA clone encoding a cyclin B gene from Nicotiana tabacum. The full-length cDNA had an open reading frame of 1422 bp with a deduced amino acid sequence of 473 residues. In order to investigate the role of cyclin B gene in the plant growth and development, transgenic tobacco overexpressing cyclin B gene was constructed. Cyclin B transgenic plants showed longer root hairs than the wild type. RT-PCR analysis revealed that expression of both cyclin B and CDK genes in transgenic plants were enhanced. Also, flow cytometry analysis showed that the isolated cells from root hair elongation zone in transgenic plants had increased G2-M phase of cell cycle compared with wild type.
