Abstract
We present the real-time imaging system to image the ion uptake in culture solution to root in dark condition, as well as from root to up-ground part in light condition. Successive imaging from dark to light condition was able to perform for the first time, which is the great advantage of using radioisotopes. A culture solution containing 32P labeled (500kBq/ml) was supplied to Lotus Japonicus. To supply sufficient light to the up-ground part, 100 LED was fixed at the up-ground part of the sample holder. The beta-rays emitted from the sample was converted to light by a CsI scintillator and was guided to a CCD camera. The image was accumulated every 3 minutes for 40 to 60 hours. The accumulation rate of 32P in root was rather constant, whereas in leaves, the rate was increased during day and decreased at night.
