日本植物生理学会年会およびシンポジウム 講演要旨集
第51回日本植物生理学会年会要旨集
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Analysis of suppressor mutants of a PARN deficient mutant, ABA hypersensitive germination2-1
*Hirayama TakashiUshiyama ShouNarusaka MariNakashita HideoNarusaka YoshihiroHayashi Shimpei
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p. 0023

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ABA hypersensitive germination2-1 shows pleiotropic phenotypes including an ABA hypersensitivity, an SA hypersensitivity, and a dwarf phenotype. The ahg2-1 mutation seems to reduce the expression of the polyA specific ribonuclease. To address the molecular basis of the AHG2/PARN function and the effect of the ahg2-1 mutation, we conducted a genetic screen for suppressor mutants of ahg2-1 using root elongation as a physiological marker. We isolated a dozen of candidates with various suppressor strengths. Interestingly, most of the suppressor mutants suppressed all the ahg2-1 phenotypes; ABA and SA hypersensitivities, and dwarf phenotype, suggesting that the functions of these suppressors and AHG2/PARN are closely related. We identified the corresponding gene for a suppressor mutant. We will discuss the function of the suppressor and the mechanisms by which ahg2-1 causes such the diverse and complicated effects.
Nishimura et al., Plant J., 44, 972-, 2005.
Nishimura et at., Plant Cell Physiol., in press.
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