抄録
Questions and anxieties to application of bioremediation to contaminating subsurface are as follows: accurate prediction of rehabilitation period, evidence of existence of contaminant－degrading microorganisms, information of growth of pathogen by supplement of nutrient to subsurface, and information of changing in microflora after bioremediation. To solve these queries, we performed development of novel simulation model to estimate completion of remediation work and DNA microarray for detection of tetrachloroethylene (PCE)－dehalogenating microorganisms. PCR－DGGE method was also applied to predict existence of kerosene－degrading bacteria and to monitor microflora change before and after bioremediation. Effect of pathogen on bioremediation was conducted using Escherichia coli as a model hygienic bacteria. These results are summarized.
