抄録
The preventive measures against poliomyeitis (polio) have not been established for about 40 years since the causative agent had been isolated. Polio vaccine is still a pending problem. Some investigators said it was effective, while others claimed not, with sometimes harmful effect to human beings. There are at least three kinds of polio viruses. Whereas it should be prepared from all kinds of viruses. The purpose of this study was to determine if the vaccine was effective in animal experiments. The vaccine inactivated by formalin was prepared from. the brain and spinal cord of mice infected with the Lansing strain. It was proved in advance that the virus was inactivated within 3 days with 0.2% formalin. The sterility, viability and safety tests were performed. The potency of the vaccine might depend, on the one hand, on the virulence of infected brains, and on the other hand, on the dose of the vaccine. The more the dose of vaccine was given, the more the mice come to survive through intracerebral challenge with virus of 4.5-14.4 times LD50.
