We prepared the mucoprotein-like substance from ox-bile. It strongly inhibits the influenza virus (PR 8 strain) hemagglutination, even in dilution of 1:128, 000 or 1:256, 000; it is heat stabile at 100°C. 15′.
This substance is digested by active influenza virus, filtrates of V. cholerae, Dip. pneumoniae, and Cl. welchii. After digestion, it loses its inhibitory activity. However, it is not digested by virus heated to 52°C for 30 minutes, hyaluronidase, and NAIO₄. Mucin from the umbilical cord does not have inhibitory activity.
The inhibitory activity exhibited by this substance in the multiplication of influenza virus in chick embryos is noticeable, but still in some degree.
As to the substance itself, we made the assumption that it perhaps has the nature of a mucoprotein.

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In a previous communication it was reported that Japanese B encephalitis virus was combined in vitro with the specific virus-neutralizing antibodies of the hyperimmune horse sera (Kawakita & Tazaki). Curiously enough, there seemed to be no absorption or blocking effect to antibodies contained in the sera of animal other than horses. According to the presents author's opinion, the seemingly qualitative species difference lies in the firmness of the antibody-virus combination. Antibodies in horse sera combine exceptionally firmly with the virus in vitro, so that blocking effect can be readily observed, irrespective of the methods of inactivation of virus, whereas with the sera of rabbits, mice, etc., heat Inactivation causes the dissociation of the virus-antibody combination once actually established. Experiments which led to the above conclusion were presented in detail

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(1) The neutralizing antibodies examination
The neutralizing antibodies in the serum of guinea pigs by the inoculation of measles virus into the testicles, did not appear on the forth day after the inoculation yet but on the seventh, for the first time, they appeared, They got to the maximum between the tenth and fifteenth, then they decreased a little on the thirtieth.
(2) The reinoculation examination
The guinea pigs showing specific pneumonia interstitialis productiva acuta, by the inoculation of measles virus which recognized with alveolitis interstitialis, peribronchiolitis and cluster of plasma cells around the small vessels, were agarn inoculated with measles virus after about five months when the various changes of their lung tissues, above mentioned, disappeared and returned to the normal condition.
This time, no remarkable changes of lung tissues from the first inoculation such as alveolitis interstitialis and peribronchiolitis were seen, but a prominent change of the cluster of plasma cells around the small vessels.
This specific phenomenon are not showing any inflammation but just a protective reaction of the lung tissues.

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Many mice were inoculated with virus (the mouse-adapted Mochizuki-strain dengus virus) intracerebrally. At various times after the inoculation, 8-10 mice were picked up at random and were injected with P³²-solution intraperitoneally, then half of the mice (4-5) were sacrificed 6 hours, and the other half 24 hours, respectively, after the P-administration, by bleeding through carotis-arteriae. Of each group, the brains were removed and pooled, and the phosphorus compounds contained were separated into fractions of acid-soluble phosphorus, phospholipid, phosphoprotein and nucleic acids, by Schneider's method. Each fraction thus obtained was subjected to the phosphorus-determination (by Allen's method) as well as radioactivity-measurement (with the GeigerMüller tube). As a control, the same tests were conducted with the materials from mice injected with a normal-mouse-brain-emulsion intracerebrally. Results obtained were as follows: (1) No definite differences were evidenced between the total phosphorus quantity of each group. (2) The radioactivities of the acidsoluble phosphorus fractions were higher in the infected groups than in the control groups. The significance was already apparent during the early stage of the murine infection, and became more distinguished with the progress of time. (3) The radioactivities of the phosphoprotein and nucleic acids fractions increased during the acme stage of the infection, while those of the control groups remained almost constant. It was concluded from these results that the permeability of the blood-liquor-barrier, as well as the metabolism centering around phosphoprotein and nucleic acids, rose distinguishedly in the organs of the virus-infected mice.

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The preventive measures against poliomyeitis (polio) have not been established for about 40 years since the causative agent had been isolated. Polio vaccine is still a pending problem. Some investigators said it was effective, while others claimed not, with sometimes harmful effect to human beings. There are at least three kinds of polio viruses. Whereas it should be prepared from all kinds of viruses. The purpose of this study was to determine if the vaccine was effective in animal experiments. The vaccine inactivated by formalin was prepared from. the brain and spinal cord of mice infected with the Lansing strain. It was proved in advance that the virus was inactivated within 3 days with 0.2% formalin. The sterility, viability and safety tests were performed. The potency of the vaccine might depend, on the one hand, on the virulence of infected brains, and on the other hand, on the dose of the vaccine. The more the dose of vaccine was given, the more the mice come to survive through intracerebral challenge with virus of 4.5-14.4 times LD50.

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