抄録
Many mice were inoculated with virus (the mouse-adapted Mochizuki-strain dengus virus) intracerebrally. At various times after the inoculation, 8-10 mice were picked up at random and were injected with P32-solution intraperitoneally, then half of the mice (4-5) were sacrificed 6 hours, and the other half 24 hours, respectively, after the P-administration, by bleeding through carotis-arteriae. Of each group, the brains were removed and pooled, and the phosphorus compounds contained were separated into fractions of acid-soluble phosphorus, phospholipid, phosphoprotein and nucleic acids, by Schneider's method. Each fraction thus obtained was subjected to the phosphorus-determination (by Allen's method) as well as radioactivity-measurement (with the GeigerMüller tube). As a control, the same tests were conducted with the materials from mice injected with a normal-mouse-brain-emulsion intracerebrally. Results obtained were as follows: (1) No definite differences were evidenced between the total phosphorus quantity of each group. (2) The radioactivities of the acidsoluble phosphorus fractions were higher in the infected groups than in the control groups. The significance was already apparent during the early stage of the murine infection, and became more distinguished with the progress of time. (3) The radioactivities of the phosphoprotein and nucleic acids fractions increased during the acme stage of the infection, while those of the control groups remained almost constant. It was concluded from these results that the permeability of the blood-liquor-barrier, as well as the metabolism centering around phosphoprotein and nucleic acids, rose distinguishedly in the organs of the virus-infected mice.
