抄録
The virus of Russian spring summer encephalitis (RSSE) multiplied without any cytopathogenic effect (CPE) in cell culture of bovine embryonic kidney. Newcastle disease (ND) virus proliferated with CPE in this cell culture, but was not able to proliferate and produce CPE in cell culture previously infected with RSSE virus. With small doses of RSSE virus it took about 4 days for the majority of cells in culture to acquire the ability to interfere with ND virus. Interfering activity of RSSE virus could be detected earlier with a smaller inoculum of ND virus than with a larger one, but the inoculum size of ND virus did not affected the result when ND virus was given at a certain interval following RSSE virus inoculation or later.
Interference between these two viruses were observed in renal cell cultures of the swine, cat, rabbit, and guinea pig, and not in cell cultures of hamster and chicken kidneys or chick embryo tissues. RSSE virus interfered with fowl plague virus and almost or entirely not with viruses of vaccinia, herpes, equine abortion, Venesuelan equine encephalitis, and bovine infectious rhinotracheitis, and HVJ (hemagglutinating virus of Japan).
Japanese encephalitis virus and Negishi virus also interfered with ND virus in bovine embryonic renal cell culture.
Interference of RSSE virus with ND virus was specifically neutralized by immune serum to RSSE virus.
Based on these findings a procedure was established for detecting and measuring RSSE virus and its neutralizing antibody.
