抄録
Myc. sp. Jucho in logarithmic phase was infected by phagus choremis, Hauduroy, and was incubated with vigorous aeration for about 24 hours at 37°C. The titer of thee lysate reached usually 1-2×1011 particles per ml. Phage was concentrated by the three times centrifugation at 17, 000rpm for 60 minutes and was carefully purified by the DNase and RNase treatments.
This phage particle has the cocoon shaped head of ca 95×150mμ in size and tail of 180-190mμ in length.
Extraction of DNA was caried out following the Mandell et al's phenol method.
It was revealed that phagus choremis did not conain RNA, and DNA content per particle was calculated as about in the same order as T-even phage.
The base composition of this preparation was determined by the quantitative paper chromatography showing the base ratio of G:A:C:T=33.44:17.21:33.44:15.91. Ratio of (A+T)/(G+C) was quite similar to that of the host cell.
Relation of the base compositions between host cell and phage was discussed.
