ミジンコ類急性遊泳阻害試験のための仔ミジンコ自動分取装置の開発

抄録

“Daphnia sp. acute immobilisation test” is a typical ecotoxicity test for chemicals. In the test procedures, the most troublesome operation is separating and collecting hundreds of water fleas in preparing the test. In this study, the automatic water flea separator and collector have been developed to simplify the test. Using the separator, 95% of the population of targeted individual Daphnia were separated from the untargeted Daphnia. Using the collector, the separated individual water fleas were collected in groups of 5 (average: 5.2, standard deviation: 0.8). The toxicity tests for potassium dichromate were performed and the efficacy of the device was evaluated. According to the results of the Daphnia sp., Acute Immobilisation Test, major damage was not observed in the individual Daphnia separated and collected automatically.