Tributyltinchloride (TBTCl) resistant bacteria were isolated from coastal sea water. Vibrio sp. M-1 was the most resistant. This strain could grow in the medium containing 40 mg/ml of TBTCI. Much uptake of TBTCI by this strain was observed in a short incubation period. However, uptake of TBTCI decreased after log phase, indicating exclusion of tin in log phase. When the strain was cultured with TBTCI, two polypeptides of 30 KDa and 12 KDa were induced.