Role of Gap Junctional Intercellular Communication (GJIC) through p38 and ERK1/2 Pathway in the Differentiation of Rat Neuronal Stem Cells

Se-Ran YANG; Sung-Dae CHO; Nam-Shik AHN; Ji-Won JUNG; Joon-Suk PARK; Eun-Hye JO; Jae-Woong HWANG; Ji-Youn JUNG; Tae-Yung KIM; Byoung-Su YOON; Bong-Hee LEE; Kyung-Sun KANG; Yong-Soon LEE

doi:10.1292/jvms.67.291

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Role of Gap Junctional Intercellular Communication (GJIC) through p38 and ERK1/2 Pathway in the Differentiation of Rat Neuronal Stem Cells

Se-Ran YANG, Sung-Dae CHO, Nam-Shik AHN, Ji-Won JUNG, Joon-Suk PARK, Eun-Hye JO, Jae-Woong HWANG, Ji-Youn JUNG, Tae-Yung KIM, Byoung-Su YOON, Bong-Hee LEE, Kyung-Sun KANG, Yong-Soon LEE

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Se-Ran YANG
Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University
Sung-Dae CHO
Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University
Nam-Shik AHN
Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University
Ji-Won JUNG
Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University
Joon-Suk PARK
Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University
Eun-Hye JO
Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University
Jae-Woong HWANG
Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University
Ji-Youn JUNG
Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University
Tae-Yung KIM
Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University
Byoung-Su YOON
Laboratory of Genetics, Department of Biology, College of Natural Science, Kyonggi University
Bong-Hee LEE
Department of Anatomy and Neurobiology, College of Medicine, Institute of Medical Science, Cheju National University
Kyung-Sun KANG
Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University
Yong-Soon LEE
Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University

Keywords: gap junctional intercellular communication, neuronal stem cell, PD98059 MEK inhibitor, rat, SB203580 p38 MAP kinase inhibitor

JOURNAL FREE ACCESS

2005 Volume 67 Issue 3 Pages 291-294

DOI https://doi.org/10.1292/jvms.67.291

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Abstract

Gap junctional intercellular communications (GJIC) contributes to neural function in development and differentiation of CNS. In this study, we have investigated the expression of GJIC during the differentiation of neuronal stem cells and 12-Ø-tetradecanoylphorbol-13-acetate (TPA)-induced neuronal stem cell-derived cells from rat brain. During neuronal stem cell differentiation, expressions of Cx43 and 32 were increased for the duration of 72 hr, however the effect were decreased on the 7d. In the neuronal stem cell-derived cells, pretreatments with p38 MAP kinase inhibitor, SB203580, and MEK inhibitor, PD98059, could protect GJIC against TPA-induced inhibition of GJIC. Our data suggest that GJIC plays an important role during neuronal stem cell differentiation, and ERK1/2 and p38 MAP kinase signaling pathway may be closely related functionally to regulate gap junction in rat neuronal stem cell-derived cells.

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