Characterization of CD34⁺ Cells from Canine Umbilical Cord Blood, Bone Marrow Leukocytes, and Peripheral Blood by Flow Cytometric Analysis

Abstract

Characterization of CD34⁺ cells in canine bone marrow, umbilical cord blood, and peripheral blood was performed by flow cytometric analysis. The ratio of CD34⁺CD45^hi cells, which are absent in human blood, was high in the CD34⁺ cell fraction, but 98% of these was suggested B-cells. The remaining CD34⁺CD45^lo cells may comprise canine hematopoietic progenitor cells, and these cells accounted for 0.23 ± 0.07% of the fraction in cord blood, 0.30 ± 0.07% in bone marrow, and 0.02 ± 0.01% in peripheral blood.