Reticulated Platelet Levels in Whole Blood and Platelet-Rich Plasma of Dogs with Various Platelet Counts Measured by Flow Cytometry

Haruhiko MARUYAMA; Hirozumi YAMAGAMI; Toshihiro WATARI; Rui KANO; Yasuhito FUJINO; Hajime TSUJIMOTO; Atsuhiko HASEGAWA; Hiroshi KAMATA

doi:10.1292/jvms.71.195

Clinical Pathology

Reticulated Platelet Levels in Whole Blood and Platelet-Rich Plasma of Dogs with Various Platelet Counts Measured by Flow Cytometry

Haruhiko MARUYAMA, Hirozumi YAMAGAMI, Toshihiro WATARI, Rui KANO, Yasuhito FUJINO, Hajime TSUJIMOTO, Atsuhiko HASEGAWA, Hiroshi KAMATA

著者情報

キーワード: canine, flow cytometry, platelet-rich plasma, reticulated platelets, whole blood

ジャーナルフリー

2009 年 71 巻 2 号 p. 195-197

DOI https://doi.org/10.1292/jvms.71.195

詳細

抄録

Reticulated platelets (RP) are young platelets that contain residual RNA, and measurement of RP has been to assess thrombopoiesis. In the present study, flow cytometric counts of RP were compared using paired specimens elicited from dogs with various platelet counts by different RP collection procedures, the whole blood method (WBM) and platelet rich plasma method (PRPM). The flow cytometric counts of RP for the specimens collected by WBM showed good and stable agreement with those taken by PRPM from the same canine subjects. The result revealed that WBM, as well as PRPM, can be used clinically to determine RP levels in dogs with abnormal platelet counts.

責任著者(Corresponding author)

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