Comparisons of Internal Behavior after Exposure to Flavobacterium psychrophilum between Two Ayu (Plecoglossus altivelis altivelis) Strains Showing Different Cumulative Mortality to Bacterial Cold Water Disease

Tetsushi KAGEYAMA; Tomonori KUWADA; Kenichi OHARA; Aya NOUNO; Tetsuya UMINO; Shuichi FURUSAWA

doi:10.1292/jvms.13-0047

Immunology

Comparisons of Internal Behavior after Exposure to Flavobacterium psychrophilum between Two Ayu (Plecoglossus altivelis altivelis) Strains Showing Different Cumulative Mortality to Bacterial Cold Water Disease

Tetsushi KAGEYAMA, Tomonori KUWADA, Kenichi OHARA, Aya NOUNO, Tetsuya UMINO, Shuichi FURUSAWA

著者情報

Tetsushi KAGEYAMA
Gifu Prefectural Agricultural Policy Division Office for the Promotion of Fisheries, 2–1–1Yabutaminami, Gifu, Gifu 500–8570, Japan
Department of Molecular and Applied Biosciences, Graduate School of Biosphere Sciences, Hiroshima University, 1–4–4 Kagamiyama, Higashi-hiroshima, Hiroshima 739–8528, Japan
Tomonori KUWADA
Gifu Prefectural Research Institute for Freshwater Fish and Aquatic Environments, Kawashima-kasada, Kakamigahara, Gifu 501–6021, Japan
Kenichi OHARA
Gifu Prefectural Research Institute for Freshwater Fish and Aquatic Environments, Kawashima-kasada, Kakamigahara, Gifu 501–6021, Japan
Aya NOUNO
Department of Molecular and Applied Biosciences, Graduate School of Biosphere Sciences, Hiroshima University, 1–4–4 Kagamiyama, Higashi-hiroshima, Hiroshima 739–8528, Japan
Tetsuya UMINO
Department of Molecular and Applied Biosciences, Graduate School of Biosphere Sciences, Hiroshima University, 1–4–4 Kagamiyama, Higashi-hiroshima, Hiroshima 739–8528, Japan
Shuichi FURUSAWA
Department of Molecular and Applied Biosciences, Graduate School of Biosphere Sciences, Hiroshima University, 1–4–4 Kagamiyama, Higashi-hiroshima, Hiroshima 739–8528, Japan

キーワード: agglutination reaction, bacterial count, Flavobacterium psychrophilum, Plecoglossus altivelis altivelis, strain-dependent

ジャーナルフリー

2013 年 75 巻 12 号 p. 1571-1575

DOI https://doi.org/10.1292/jvms.13-0047

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詳細

抄録

Bacterial cold water disease (BCWD) in ayu (Plecoglossus altivelis altivelis) has a serious impact on aquaculture and fisheries. There is known to be a significant difference among ayu strains with regard to mortality caused by BCWD. In this study, the immune response of different ayu strains against Flavobacterium psychrophilum infection was observed. One strain was resistant to infection by F. psychrophilum, and the other was susceptible to infection by the same bacteria. The number of bacteria in the body was observed in each ayu strain, and the change in bacterial counts was similar. However, there was a significant difference in bacterial count in the spleen between the two strains on days 6, 9, 12 and 15 after exposure. To observe the immune response against F. psychrophilum, agglutination assay using serum was performed. An agglutination reaction in the resistant ayu strain was observed in 4 out of 6 ayu on day 6 after exposure, while no reactions in the susceptible ayu strain were observed in any sampled fish until day 12. However, some reactions in the susceptible ayu strain were observed in surviving ayu. These results indicate that there is a correlation between the presence of bacterial multiplication and agglutination reaction against F. psychrophilum.

責任著者(Corresponding author)